Ciro Gianmaria Amoroso

Experienced researcher specializing in molecular biology and plant-applied biotechnologies. Proven track record in identifying and characterizing genes responsible for biotic and abiotic stress responses. Skilled in utilizing transcriptomic profile analysis and genome editing techniques to gain valuable insights into gene function. Passionate about advancing scientific knowledge and actively contributing to the development of innovative solutions in the field of plant genetics.

• Amoroso C.G. and Andolfo G. 2024. SiMul-db: a database of single and multi-target Cas9 guides for hazelnut editing. Front. Genet. 15:1467316. doi: 10.3389/fgene.2024.1467316
• Evangelista A.R.*, Amoroso C.G.*, Nitride C., Andolfo G. 2024. Seed storage allergens tackled via next-generation research assistant. Front. Food. Sci. Technol, 4:1372770. doi: 10.3389/frfst.2024.1372770
  *These authors share first authorship
• Amoroso C.G., D’Esposito D., Aiese Cigliano R., Ercolano M.R. 2023. Comparison of Tomato Transcriptomic Profiles Reveals Overlapping Patterns in Abiotic and Biotic Stress Responses. Int. J. Mol. Sci, 24, 4061. https://doi.org/10.3390/ijms24044061
• D’Esposito D., Guadagno A., Amoroso C.G., Cascone P., Cencetti G., Michelozzi M., Guerrieri E., Ercolano M.R. 2023. Genomic and metabolic profiling of two contrasting tomato cultivars for tolerance to Tuta absoluta. Planta, 257-47. https://doi.org/10.1007/s00425-023-04073-8
• Amoroso C.G., Andolfo G., Capuozzo C., Di Donato A., Martinez C., Tomassoli L., Ercolano M.R. 2022. Transcriptomic and genomic analysis provides new insights in molecular and genetic processes involved in zucchini ZYMV tolerance. BMC genomics 23, 371. https://doi.org/10.1186/s12864-022-08596-4
• García J.C., Guadagno A., Paytuvi-Gallart A., Saera-Vila A., Amoroso C.G., D'Esposito D., Andolfo G., Aiese Cigliano R., Sanseverino W., Ercolano M.R. 2022. PRGdb 4.0: an updated database dedicated to genes involved in plant disease resistance process. Nucleic Acids Research, Volume 50, Issue D1, 7. Pages D1483– D1490. https://doi.org/10.1093/nar/gkab1087
• Giordano M., Amoroso C.G., Rouphael E.Y., De Pascale S., Cirillo C. 2020. An appraisal of biodegradable mulch films with respect to strawberry crop performance and fruit quality. Horticulture, 6 (3), p. 48. https://doi.org/10.3390/horticulturae6030048
• Chikh-Rouhou H., Lohwasser U., Picó Sirvent M.B., Flores León A., Martínez S.G., GuadagnoA., Amoroso C.G., Ercolano M.R. 2023. Cucurbitlocal – A Collaborative Initiative to Strengthen Valorization of Cucurbita Local Germplasm for Sustainable Agriculture. Cucurbit Genetics Cooperative Report, 46. ISSN 2689-1719
• Amoroso C.G., Panthee D.R., Andolfo G., Ramìrez F.P., Ercolano M.R. 2022. Genomic Tools for Improving Tomato to Biotic Stress Resistance. In: Genomic Designing for Biotic Stress Resistant Vegetable Crops. Kole, C. (eds) Genomic Designing for Biotic Stress Resistant Vegetable Crops. Springer, Cham. https://doi.org/10.1007/978-3-030-97785-6_1
• Di Matteo A., Castaldo C., Amoroso C.G., Ercolano M.R. 2022. Geni e funzioni geniche per il miglioramento della tolleranza agli stress biotici e abiotici delle piante agrarie. In: Recenti Progressi Nella Genetica Agraria. Frusciante L. Corrado G. (eds) Idelson Gnocchi. ISBN: 978-88-79477901
• Andolfo G., Amoroso C.G., Ercolano M.R. 2021. Disease Resistance Breeding with Genomic Tools in Zucchini (Cucurbita pepo L.). In: Al-Khayri J.M., Jain S.M., Johnson D.V. (eds) Advances in Plant Breeding Strategies: Vegetable Crops. Springer, Cham. https://doi.org/10.1007/978-3-030-66961-4_11

• Supported practical exercises on mitosis and meiosis, breeding, nucleic acid extraction, PCR, and electrophoresis as part of the “Genetica Vegetale Applicata” course (6 ECTS, code U0015) and the Bachelor's Degree in “Scienze e Tecnologie Erboristiche” at the University of Naples Federico II. Academic Years:2021/2022,2022/2023
• Assisted with bioinformatics exercises on genomic and transcriptomic data analysis, using bioinformatics software (Blast, CD-search, InterProScan) in the “Laboratorio di Genomica e Trascrittomica di organismi di Interesse Agrario” course (6 ECTS, code U1847 -39925) for the Bachelor's Degree in “Agricultural, Forestry, and Environmental Sciences” and the Master's Degree in “Agro-Environmental Biotechnology” at the University of Naples Federico II. Academic Years:2021/2022,2022/2023
• As a Research Fellow, provided tutoring to thesis students and Ph.D. candidates

• January2021- September2021: Laboratory of Professor Antonio Granell, IBMCP (UPV-CSIC), Valencia, Spain. Objectives: assembly of bacterial and viral vectors for silencing and overexpressing selected genes. Stable and transient transformations of tomato plant flowers.
• January2020 - May2020: La Semiorto Sementi, Sarno, Salerno, Italy. Objectives: breeding and phenotyping techniques for company-relevant plants.
• January2018 - May2018: ADESVA – ADESVA – Centro Tecnológico de la Agroindustria, Lepe, Huelva, Spain. Objective: data collection, study, and characterization of strawberry varieties grown using biodegradable mulching films.
• September2017 - June2018: University of Huelva, Department of Agricultural Engineering, Huelva, Spain. Objective: training period as part of the Erasmus+ program.

• Technical Staff at the University of Naples Federico II. Start Date:21/09/2023 - Present
• Research Fellow on the project "HARNESS-TOM: Harnessing the Value of Tomato Genetic Resources for Now and the Future". Start Date:01/05/2022 –20/09/2023

• Professor Andolfo Giuseppe – Study of seed storage protein genes involved in allergenicity using transcriptomic and molecular approaches.
• Professor Ercolano Maria – Ph.D. project aimed at improving tomato plant responses to biotic and abiotic stresses through genomic and biotechnological approaches.
• Professor Granell Antonio (IBMCP, Spain) – Utilization of biotechnological approaches to accelerate tomato plant flowering. Collaboration within the HARNESS-TOM project.
• Dr. Sanseverino Walter and Dr. Aiese Cigliano Riccardo (Sequentia Biotech) – Transcriptomic analyses to identify genes involved in responses to biotic and abiotic stresses.
• Dr. Guerrieri Emilio (Research Director - IPSP-CNR) – Identification of genes and metabolites involved in Tuta absoluta tolerance.
• ADESVA and Professor De Pascale Stefania – Data collection and analysis on the use of biodegradable mulching films.